Zeiss Lattice Light-Sheet microscope

Applications

Lattice light-sheet (LLS) microscopy technology was developed by the laboratory of Nobel Prize winner Eric Betzig and was the subject of a very first publication in the journal Science in 2014. This technology allows 4D imaging (also called volumetric imaging), i.e. to observe living samples with a high spatial resolution (allowing to visualize subcellular structures) combined with a very high temporal resolution, with low phototoxicity and low photobleaching. While the first versions of LLSM were very heavy to use, Zeiss made a very user-friendly version of this technology. Zeiss Lattice Lightsheet 7 allows gentle volumetric imaging at high speed (e.g. 1 cell volume acquired in less that 1 second, depending on desired resolution and number of channels) and near-isotropic spatial resolution (approx. 350-400 nm). Of note, samples must be of reasonable thickness (< 200 µm).

Note: Volumetric high-resolution imaging produces large amounts of data (Gb to Tb!) in a very short amount of time. Projects must be discussed with platform specialists regarding data storage and data analysis. Please, contact us.

Technical description

Zeiss Lattice Lightsheet 7, inverted configuration, fully motorized stage
Excitation laser lines (diodes): 488 nm (10 mW), 561 nm (10 mW) and 640 nm (5 mW)
Detectors: None
Objectives: special optics developed by Zeiss specifically for lattice light-sheet, water auto-immersion system for long-term imaging. Note: stage and optics allow imaging in any high quality (#1.5) glass-bottom cell culture dishes (chambers such as Ibidi µ-slide or LabTek, 35 mm diameter Petri dishes, 24 or 96-well dishes…).
Drift correction: fully controlled stage
UV-Vis light source: None
Camera: PCO.edge 4.2 sCMOS camera (QE 82%, pixel size 6.5 µm x 6.5 µm, resolution 2048 x 2048)
Incubator: integrated (control of CO2, temperature and humidity; possibility to add N2 supply to work in hypoxic conditions)
Antivibration table (compressed air)